| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 141 | Bordetella avium | AAGACGGCACCGAAGACA | AAACGTCCAGCTTGGCCA | 58.87 | 59.81 | 192 | 113 |
100.00%
|
100.00%
|
| Primer 142 | Bordetella avium | AGACGGCACCGAAGACATC | AAACGTCCAGCTTGGCCA | 59.78 | 59.81 | 191 | 113 |
100.00%
|
100.00%
|
| Primer 143 | Bordetella avium | TTTCGCGTTCGTTGCTCC | ACGCATGTGCAAACTCCG | 59.07 | 59.05 | 217 | 113 |
100.00%
|
100.00%
|
| Primer 144 | Bordetella avium | TCCGCAATTCAGTGCCCA | GCGCACATTGAGGTTGGTG | 59.57 | 60.08 | 199 | 113 |
100.00%
|
100.00%
|
| Primer 145 | Bordetella avium | TCCGCAATTCAGTGCCCA | TGCGCACATTGAGGTTGG | 59.57 | 58.65 | 200 | 113 |
100.00%
|
100.00%
|
| Primer 146 | Bordetella avium | TCACCGCTCCGCTCATTAC | TGATGCCGTTACTGCGCA | 59.86 | 60.05 | 285 | 113 |
100.00%
|
100.00%
|
| Primer 147 | Bordetella avium | TCGAAACGCCTGGCCATT | CCGCTTTCACTGGGCAGTA | 59.97 | 60.00 | 208 | 112 |
100.00%
|
100.00%
|
| Primer 148 | Bordetella avium | TCGAAACGCCTGGCCATT | TGCATCCATACGGTCACGG | 59.97 | 59.86 | 269 | 112 |
100.00%
|
100.00%
|
| Primer 149 | Bordetella avium | TCGAAACGCCTGGCCATT | GCCTGCATCCATACGGTCA | 59.97 | 59.85 | 272 | 112 |
100.00%
|
100.00%
|
| Primer 150 | Bordetella avium | AACAGCAGCCAAGTCCGT | TCGACAAACCCATGCGCA | 59.49 | 60.28 | 281 | 112 |
100.00%
|
100.00%
|